Emerging biosensors to detect aflatoxin M1 in milk and dairy products

Aflatoxin M-1 (AFM(1)) is an important risk factor threatening the safety of milk and dairy products due to its carcinogenic and teratogenic effects on humans. To prevent AFM(1) from causing damage to human health, developing reliable methods to monitor its levels in milk and dairy products is of great importance. Biosensors built with recognition and detection systems have attracted extensive attention for their simplicity, portability, sensitivity, and selectivity. The commonly developed biosensors for detecting AFM(1) are antibody-based sensors (immunosensors) and aptamer-based sensors (aptasensors). This review focused on the advances of immuno-sensors, aptasensors, and other emerging receptors-based sensors for the detection of AFM(1) in milk and dairy products in the past five years. These biosensors were reviewed with representative examples according to their signal transduction systems, mainly including colorimetry, fluorescence, electrochemistry, and others. The unique advantages and drawbacks of immunosensor and aptasensor, and the future opportunities and challenges were also discussed.