In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht). A comparison of the physico-chemical and functional characteristics of the Cht/KC/AgNPs film was performed with those of Cht, Cht/KC, and Cht/AgNPs. SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 mu m) compared to AgNPs (20-80 nm). The FTIR analysis suggested that the interactions between Cht and AgNPs were stronger than those between Cht and KC. The tensile strength of Cht film increased from 16 MPa to similar to 24 MPa in Cht/KC/AgNPs film. The introduction of KC and/or AgNPs considerably improved the light and moisture barrier capacity of the Cht film. The UV light transmittance decreased by 50 % for Cht film when incorporated by KC and AgNPs. Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity. In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs. For the packaging properties, all the films were tested for their ability to keep the freshness of apple slices as wrapping material. The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices. Moreover, the Cht/KC/AgNPs film exhibited a migration of silver meeting the standards set by EFSA and ECHA, which makes this film safe for food packaging.
