Non-specific signals in real-time RT-PCR for detecting respiratory viruses

被引：1
|
作者：
Mak, Gannon C. K. [1 ]
Hung, Derek L. L. [1 ]
机构：
[1] Ctr Hlth Protect, All Microbiol Div, Publ Hlth Lab Serv Branch, Dept Hlth, Hong Kong, Peoples R China
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2024年 / 108卷 / 03期
关键词：
PCR; Non-specific signals; Reagent lot;
D O I：
10.1016/j.diagmicrobio.2023.116163
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We describe our experiences in investigating the origin of non-specific signals during the development phase of a multiplex PCR assay for respiratory viruses. After ruling out various sources of error, eventually we discovered the non-specific signal was related to the particular lot of the PCR kit.
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页数：3
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