DNA barcoding of rotifers along the Cochin backwaters, southwest coast of India

被引：0
|
作者：
Ravi, Asha [1 ,2 ]
Sukumaran, Sandhya [1 ]
Varghese, Molly [1 ]
Raj, Neenu [1 ]
机构：
[1] Cent Marine Fisheries Res Inst, Ernakulam North, Kochi 682018, India
[2] Cochin Univ Sci & Technol, South Kalamassery, Kochi 682022, India
来源：
CURRENT SCIENCE | 2023年 / 124卷 / 03期
关键词：
Backwaters; DNA barcoding; partial sequen-ces; pure culture; rotifer; BRACHIONUS-CALYCIFLORUS; GENETIC DIFFERENTIATION; PLICATILIS; SPECIATION; AMPLIFICATION; PHYLOGENY; DIVERSITY; EVOLUTION; PRIMERS; COMPLEX;
D O I：
10.18520/cs/v124/i3/327-332
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
This study examines the DNA barcoding of rotifers from the Cochin backwaters, Kerala, India, with specimens obtained from the pure culture, using primers LCO1490/ HCO2198 and a recently reported primer 30F/885R which was designed especially for the rotifers. Partial sequences of the mitochondrial COI gene from five spe-cies belonging to three genera (Brachionus, Keratella and Asplanchna) and two families (Brachionidae and Asplan-chnidae) of the order Ploimida were generated. All the obtained nucleotide sequences were submitted to NCBI (accession numbers: OL477582-OL477584, OL477586 and OL757796).
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页码：327 / 332
页数：6
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