The article considers the student's electronic portfolio as an integral part of the educational activities of a modern multidisciplinary university providing strategic communication between students, graduates, teachers and potential employers. The importance of the e-portfolio as an effective means of a comprehensive monitoring of students' achievements and the formation of positive students' motivation to acquire professional competencies necessary for successful employment and demanded in the labor market is substantiated. The aim of the article is to present ways to optimize work with the e-portfolio that reflects the totality of the results of the student's educational and cognitive activities in accordance with the individual educational trajectory and career expectations. The reliability of the research is justified by a suite of methods of scientometric analysis, content analysis and pedagogical modeling. The material of the research was scientific publications presented in the Scopus and RSCI citation databases, open data posted on 45 Russian multidisciplinary university websites, instructions for electronic portfolios of students, as well as texts of vacancies of the two largest online recruiting portals. The authors identified the main trends in scientific and pedagogical research on the problems of e-portfolios, analyzed the experience of using e-portfolios in multidisciplinary universities, determined the significance of e-portfolios in employment. The authors developed a new principle of forming e-portfolios; it involves the organization of a portfolio as a hierarchy of achievements in different types of educational and cognitive activities, considering university, profile and regional specifics. The authors present a multilevel model of the e-portfolio, whose implementation includes gamification elements and allows visualizing an individual educational trajectory, and propose mechanisms of improving the efficiency of the e-portfolio in the educational activities of a multidisciplinary university. The authors conclude that there is a need for pedagogical support of students in creating, filling and editing their eportfolios. The authors express the idea that professional self-presentation in the form of a public presentation of an eportfolio should become an integral part of the state final certification. The practical significance of the study consists in the possibility to apply the proposed structure of an e-portfolio to track the dynamics of the student's personal and professional development, to correct an educational trajectory and consciously build a professional career.