Current and Emerging Diagnostic Approaches to Bacterial Diseases of Ruminants

被引：4

作者：

Loy, John Dustin ^{[1
,4
]}

Clawson, Michael L. ^{[2
]}

Adkins, Pamela R. F. ^{[3
]}

Middleton, John R. ^{[3
]}

机构：

[1] Univ Nebraska, Nebraska Vet Diagnost Ctr, Sch Vet Med & Biomed Sci, Lincoln, NE 68583 USA

[2] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA

[3] Univ Missouri, Coll Vet Med, Dept Vet Med & Surg, Columbia, MO USA

[4] Univ Nebraska, Nebraska Vet Diagnost Ctr, 4040 East Campus Loop N, Lincoln, NE 68583 USA

来源：

VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE | 2023年 / 39卷 / 01期

关键词：

REAL-TIME PCR; INFECTIOUS BOVINE KERATOCONJUNCTIVITIS; DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; CHAIN-REACTION ASSAYS; MALDI-TOF; SALMONELLA-DUBLIN; COMPONENT CAUSES; MORAXELLA-BOVIS; IDENTIFICATION;

D O I：

10.1016/j.cvfa.2022.10.006

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Emerging diagnostic approaches such as rtPCR, MALDI-TOF, and gene sequencing have enhanced diagnostic testing for a variety of infectious diseases in ruminants. rtPCR has enhanced sensitivity, reduced testing time, and minimizes the need for cul-ture. However, interpretation of result requires additional considerations. For MALDI-TOF and WGS, isolation of the pathogen in pure culture is still required. Sequence-based approaches applied directly to clinical samples hold promise for diagnostic testing for these pathogens, but advances in technology, reduction in cost, and ease of analysis need to be comparable with existing methods to be practical.

引用

页码：93 / 114

页数：22

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