Clinical evaluation of a Borrelia modified two-tiered testing (MTTT) shows increased early sensitivity for Borrelia burgdorferi but not other endemic Borrelia species in a high incidence region for Lyme disease in Wisconsin

被引：2

作者：

Sabin, Arick P. ^{[1
,2
]}

Scholze, Brooklynn P. ^{[2
]}

Lovrich, Steven D. ^{[2
]}

Callister, Steven M. ^{[2
]}

机构：

[1] Gundersen Hlth Syst, Div Infect Dis, La Crosse, WI 54601 USA

[2] Gunderson Med Fdn, Microbiol Res Lab, La Crosse, WI 54601 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2023年 / 105卷 / 01期

关键词：

Lyme disease; Borrelia burgdorferi; Antibodies; Standard two-tiered testing (STTT); Modified two-tiered testing (MTTT); LINKED-IMMUNOSORBENT-ASSAY; UNITED-STATES; DIAGNOSIS; SERODIAGNOSIS; GUIDELINES;

D O I：

10.1016/j.diagmicrobio.2022.115837

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A modified two-tiered testing algorithm (MTTT; ZEUS Scientific) for Borrelia burgdorferi was recently FDA -cleared. We evaluated the MTTT algorithm to confirm Lyme disease and compared the findings in parallel with those obtained using standard two-tiered testing (STTT). Medical records from patients who submitted sera for laboratory confirmation of Lyme-like disease were reviewed. Three hundred twenty patient samples were run by the STTT and MTTT approaches and the results compared. Positive STTT samples were also posi-tive by MTTT (94%). The MTTT confirmed the illness in 116 subjects (36%, P = 0.007), and 30 (26%) were nega-tive by the STTT. Increased MTTT sensitivity was seen (P = 0.0005) during early infection. MTTT was insufficiently sensitive to identify other non-Borrelia spp. infections. Routine adoption of MTTT would improve sensitivity for early Lyme disease attributable to B. burgdorferi, but may not capture illness attrib-uted to B. mayonii and B. miyamotoi. (c) 2022 Elsevier Inc. All rights reserved.

引用

页数：5