Otolith morphometry and Fourier transform near-infrared (FT-NIR) spectroscopy as tools to discriminate archived otoliths of newly detected cryptic species, Etelis carbunculus and Etelis boweni

Cryptic speciation was recently verified in Etelis carbunculus, an important component of federally managed bottomfish fisheries in the Pacific Territories of the United States. As a result, archived otolith collections used for fishery assessment are now contaminated with newly described E. boweni in areas where these species co-occur. We compared the efficacy of otolith morphometrics and Fourier transform near-infrared (FT-NIR) spectroscopy to discriminate species first using voucher (i.e., known species) otoliths (n = 93) from the SW Pacific, then applied optimal models to archived otoliths (n = 91) collected around Guam. Significant and distinguishable differences in otolith morphometrics as well as FT-NIR spectral absorbance patterns were observed between E. carbunculus and E. boweni voucher samples. Classification models applied using both morphometric measurements (quadratic discriminant analysis) and FT-NIR spectral data (partial least squares discriminant analysis) were able to predict species with a high (93 - 100%) degree of accuracy despite a relatively large spatial area of specimen collection ( +/- 10(degrees) latitude and longitude) and regardless of whether otoliths were whole (i.e., unbroken). Further, each method identified members of newly described E. boweni in the archived collection of E. carbunculus otoliths captured around Guam, providing strong evidence that the species' distributions overlap in this region. The purported identification of both E. carbunculus and E. boweni in the archived catch from Guam has important implications for fisheries management; therefore, it is imperative that the corresponding otolith collections are examined to ensure that the otoliths are assigned to the correct species.