Selection of frozen-thawed stallion semen by microfluidic technology

被引:4
|
作者
Vigolo, Veronica [1 ,2 ]
Gautier, Camille [1 ]
Falomo, Maria Elena [2 ]
Aurich, Christine [1 ,3 ]
机构
[1] Vetmeduni Vienna, Dept Small Anim & Horses, Artificial Inseminat & Embryo Transfer, Vienna, Austria
[2] Univ Padua, Dept Anim Med Prod & Hlth MAPS, Legnaro, PD, Italy
[3] Vetmeduni Vienna, Dept Small Anim & Horses, Artificial Inseminat & Embryo Transfer, Veterinarpl 1, A-1210 Vienna, Austria
关键词
frozen-thawed; microfluidics; sperm; stallion; SPERM SELECTION; EQUINE SEMEN;
D O I
10.1111/rda.14305
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The use of microfluidic technology is increasing in artificial reproduction technologies: With a small amount of semen, it allows for the selection of sperm with the best characteristics of kinetics, morphology and chromatin integrity. The ZyMot Multi (850 mu l) is the most popular device of ZyMot Fertility Inc. To date, it was proven to be a valid instrument for sperm selection for in vitro fertilization and intracytoplasmic sperm injection in men. The aim of this study was to test the efficacy of the ZyMot Multi (850 mu l) for stallion semen. Frozen-thawed semen from 15 stallions that were previously classified as being of 'good fertility' (GF; n = 8; pregnancy rate >= 40%) and 'poor fertility' (PF; n = 7; pregnancy rate < 20%), respectively, was used. Each ejaculate was assessed before and after microfluid recovery for kinetics (CASA), membrane integrity (MI) (SYBR14/PI), membrane functionality (MF) (HOS test), acrosome integrity (Spermac Stain Kit), morphology (Spermac stain kit), mitochondrial membrane potential (MMP) (JC-1) and chromatin integrity (aniline blue staining). Sperm concentration was reduced after sperm recovery in both groups, but more markedly in frozen-thawed semen of PF stallions (p < .05). Microfluid recovery increased total motility, MI, MF and MMP. While there was a significant increase in the percentage of progressively motile sperm after sperm microfluid recovery, there was a decrease in DAP, DSL, VAP, VSL, LIN, WOB and ALH (p < .05). A slight increase (p < .05) was detected in beat-cross frequency. The present results suggest that the ZyMot Multi (850 mu l) device selects a specific sperm population from any stallion ejaculate with motile sperm and could therefore be a valid tool for in vitro testing with the aim to predict the fertility of frozen-thawed stallion semen.
引用
收藏
页码:443 / 449
页数:7
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