Two years of nitrogen addition altered soil microbial community structure and function in a desert steppe of northern China

Context. Although nitrogen (N) deposition application has resulted in excess N entering grassland ecosystems, our understanding of how short-term N enrichment affects soil microbial community in a desert steppe is incomplete. Aims. This study assessed the effects of 2 years of N addition on structure and function of soil microbial community at the soil depth of 0-10 cm in a desert steppe.Methods. Six addition rates of N (equivalent to 0, 5, 10, 15, 20 and 40 g N m-2 year-1) were applied in a desert steppe of northern China, and the 16S rRNA and ITS 1 genes were sequenced for the prokaryotic and fungal communities, respectively.Key results. N addition altered prokaryotic taxa composition, but not fungal taxa composition and a-diversities of prokaryotic and fungal communities. N shifted microbial (3-diversities, and prokaryotic (3-diversity was more sensitive than fungal (3-diversity. Abundances of predicted prokaryotic functions decreased as N rates increased, whereas predicted fungal functions presented no obvious variations among N additions. Predicted enzyme activities related to N cycling in prokaryotes were similar to fungi, but variations of C and P cycling-related enzyme activities were opposite to fungi. Additionally, phylum abundance, a-diversity, and (3-diversity of prokaryotes were more closely influenced by soil and plant properties than fungi.Conclusions. Two years of N addition altered both microbial community structure and function, but the prokaryotic community responded more sensitive than the fungal community.