This study aimed to establish the effectiveness of slow tempo bodyweight squat combined with an isometric squat (ST-ISO), and an isometric squat alone (ISO), as a post-activation performance enhancement protocol (PAPE) for jump height improvement. The study sample consisted of 41 trained men aged 18-24. The ST-ISO group (n = 17) performed three five-second sets of the maximal voluntary back squat while pushing on an immovable bar and two sets of five repetitions of a slow-tempo (5-0-5-0) body squat immediately after isometry with a 2-m rest interval. The ISO (n = 14) group only performed isometric squats, and the control group (CG; n = 10) performed a 5-min treadmill run at 6 km/h. The countermovement jump (CMJ) height results were analyzed from the baseline and then at 3, 5, 7, and 9 min after the PAPE protocols. The statistical significance was set at p < 0.05. RM-ANOVA revealed differences in the group-minute interaction (F = 2.70; p = 0.0083; & eta;2 = 0.1243), and post-hoc tests demonstrated a significant decrease in CMJ after 5 min in the ISO group (p < 0.0446). The performance of the ST-ISO group markedly decreased in the 3rd and 7th min after PAPE (p = 0.0137; p = 0.0424, respectively), though it improved significantly in the final minute (p < 0.0030). Chi-squared analysis revealed that the ST-ISO group peaked more frequently in the 9th min (X2 = 17.97; p = 0.0214). However, CMJ height improvement did not differ between the PAPE protocols, thus it was close to statistical significance (t = -1.82; p = 0.07; ES = 0.7). The ST-ISO protocol provided jump enhancement, though the deterioration observed in the first minutes after the protocols suggest the rest period after activity requires attention, and the methods need to be individualized.
