Study of stability, kinetic parameters and release of lysozyme immobilized on chitosan microspheres by crosslinking and covalent attachment for cotton fabric functionalization

被引：4

作者：

Ceron, Annie ^{[1
]}

Costa, Silgia ^{[1
]}

Imbernon, Rosely ^{[6
]}

de Queiroz, Rayana ^{[2
]}

de Castro, Jordana ^{[3
]}

Ferraz, Humberto ^{[4
]}

Oliveira, Rodrigo ^{[5
]}

Costa, Sirlene ^{[1
]}

机构：

[1] Univ Sao Paulo, Sch Arts Sci & Humanities, Text & Fash Course, Ave Arlindo Bettio 1000,Parque Ecol, Sao Paulo, SP, Brazil

[2] Inst Technol Res Sao Paulo State, Bionanomfg Business Unit, Chem & Manufactured Prod Lab, BR-05508901 Sao Paulo, SP, Brazil

[3] Inst Technol Res Sao Paulo State, Bionanomfg Business Unit, Chem Proc & Particle Technol Lab, BR-05508901 Sao Paulo, SP, Brazil

[4] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Pharm, Ave Prof Lineu Prestes 580, BR-05508000 Sao Paulo, Brazil

[5] Univ Sao Paulo, Bauru Sch Dent, Biochem Lab, Biol Sci Dept, Alameda Dr Octavio Pinheiro Brisolla 9-75,Vila Un, BR-17012901 Bauru, SP, Brazil

[6] Univ Sao Paulo, Sch Arts Sci & Humanities, Nat Sci Grad, Ave Arlindo Bettio 1000,Parque Ecol Tiete, Sao Paulo, SP, Brazil

来源：

PROCESS BIOCHEMISTRY | 2023年 / 128卷

基金：

巴西圣保罗研究基金会;

关键词：

Antimicrobial microspheres; Lysozyme immobilization; Chitosan; Crosslinking; Covalent attachment;

D O I：

10.1016/j.procbio.2023.02.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chitosan microspheres are used in the biotechnological, biomedical, and enzyme immobilization fields. This study synthesized lysozyme-chitosan microspheres by crosslinking and covalent attachment. The synthesis conditions were adjusted, and spherical particles content lysozyme were obtained and characterized by Scanning electron microscopy (SEM) and Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Activity and stability assays were conducted to determine the immobilization method's influence, and the ki-netics parameters were calculated. The enzymatic activity recovery yields of 65.8% and 20.0% were obtained for crosslinking and covalent attachment, respectively. Surface lysozyme-chitosan microspheres' enzymatic activity was obtained by covalent attachment. By the crosslinking method, surface and into particle enzyme activity were observed. The functionalization of cotton fabric was achieved, and the impregnation slightly modified cotton fabric properties such as resistance and permeability. Finding antimicrobial properties against bacteria S. aureus and P. aeruginosa, with a growth of 49.12% and 65.31%, respectively, were obtained.

引用

页码：116 / 125

页数：10