Inflammasomes NLRP3 and AIM2 in peri-implantitis: A cross-sectional study

被引:5
|
作者
Galindo-Moreno, Pablo [1 ,2 ,8 ]
Montalvo-Acosta, Saray [3 ]
Martin-Morales, Natividad [1 ,2 ,4 ,5 ]
Carrillo-Galvez, Ana Belen [2 ]
Gonzalez-Rey, Elena [6 ]
O'Valle, Francisco [2 ,5 ,7 ]
Padial-Molina, Miguel [1 ,2 ]
机构
[1] Univ Granada, Sch Dent, Dept Oral Surg & Implant Dent, Granada, Spain
[2] Inst Invest Biosanit ibs GRANADA, Granada, Spain
[3] Univ Granada, PhD Program Clin Med & Publ Hlth, Granada, Spain
[4] Univ Granada, PhD Program Biomed, Granada, Spain
[5] Univ Granada, Sch Med, Dept Pathol, Granada, Spain
[6] Parque Tecnol Salud, Inst Parasitol & Biomed Lopez Neyra, IPBLN CSIC, Granada, Spain
[7] Univ Granada, Inst Biopathol & Regenerat Med IBIMER, CIBM, Granada, Spain
[8] Univ Granada, Sch Dent, Dept Oral Surg & Implant Dent, Campus Univ Cartuja,S-N, Granada 18071, Spain
关键词
AIM2; inflammasome; inflammation; NLRP3; peri-implant disease; peri-implantitis; 2017 WORLD WORKSHOP; MARGINAL BONE LOSS; CONSENSUS REPORT; GASDERMIN D; PERIODONTITIS; INTERLEUKIN-1-BETA; CLASSIFICATION; PYROPTOSIS; BIOMARKERS; MECHANISM;
D O I
10.1111/clr.14174
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Background: Inflammasome components NLRP3 and AIM2 contribute to inflammation development by the activation of caspase-1 and IL-1 ss. They have not been yet evaluated in samples from patients with active peri-implantitis. Thus, the aim of the present study is to analyze the expression of inflammasomes NLRP3 and AIM2 and subsequent caspase 1 and IL-1 ss assessing the microenvironment of leukocyte subsets in samples from patients with active peri-implantitis. Methods: Biopsies were collected from 33 implants in 21 patients being treated for peri-implantitis. Biopsies from gingival tissues from 15 patients with healthy periodontium were also collected for control. These tissues were evaluated through conventional histological stainings. Then, immunohistochemical detection was performed to analyze NLRP3, AIM2, caspase-1, and IL-1 ss and markers of different leukocyte subsets. PCR for inflammasomes and related genes was also done. Results: This manuscript reveals a high immunohistochemical and mRNA expression of NLRP3 and AIM2 inflammasomes, caspase-1, and IL-1 ss in biopsies collected from human peri-implantitis. The expression of the tested markers was significantly correlated with the increase in inflammatory infiltrate, probing depth, presence of biofilm, and bleeding on probing. In these peri-implantitis lesions, the area of biopsy tissue occupied by inflammatory infiltrate was intense while the area occupied by collagen was significantly lower. In comparison with periodontal healthy tissues, the inflammatory infiltrate was statistically significantly higher in the peri-implantitis biopsies and was mainly composed of plasma cells, followed by T and B lymphocytes. Conclusion: In human peri-implantitis, chronic inflammation can be explained in part by the action of IL-1 ss/ caspase 1 induced through NLRP3 and AIM2 inflammasome activation.
引用
收藏
页码:1342 / 1353
页数:12
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