The Synergistic Interaction of Arabinases of Different Types of Action in the Bioconversion of Sugar Beet Pulp and Apple Pomace

被引:1
|
作者
Semenova, M. V. [1 ]
Kuryshkina, M. S. [2 ]
Sinitsyn, A. P. [1 ,2 ]
机构
[1] Russian Acad Sci, Fed Res Ctr Fundamentals Biotechnol, Moscow 119071, Russia
[2] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia
关键词
endoarabinase; exoarabinase; arabinofuranosidase; arabinoxylan-arabinofuranhydrolase; synergism; sugar beet pulp; apple pomace; HYDROLYSIS;
D O I
10.1134/S0003683823020102
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The interaction between endoarabinase (endoA) and exo-type enzymes has been studied during their joint hydrolysis of branched arabinane (BAra), sugar beet pulp (SBP), and apple pomace (AP). It has been shown that mixtures of endoA with arabinofuranosidase (AF) or arabinoxylan-arabinofuranhydrolase (AXH) with endoA contents of 20 and 40%, respectively, were the most effective in the hydrolysis of BAra. As a result of the optimization of the complex of arabinases, cellulases, and pectinase, almost complete conversion of AP into monosaccharides (arabinose, glucose, and fructose) has been carried out. During the hydrolysis of SBP, the conversion rate of hemicellulose (arabinane) was more than 50% and for cellulose, 75%.
引用
收藏
页码:168 / 175
页数:8
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