A Comparative Study of Voltammetric vs Impedimetric Immunosensor for Rapid SARS-CoV-2 Detection at the Point-of-care

被引：3
|
作者：
Tortolini, Cristina [1 ]
Angeloni, Antonio [1 ]
Antiochia, Riccarda [2 ]
机构：
[1] Univ Roma La Sapienza, Dept Expt Med, Viale Regina Elena 324, I-00166 Rome, Italy
[2] Univ Roma La Sapienza, Dept Chem & Drug Technol, Ple Aldo Moro 5, I-00185 Rome, Italy
来源：
ELECTROANALYSIS | 2023年 / 35卷 / 04期
关键词：
Immunosensor; SARS-CoV-2; voltammetric biosensor; impedimetric biosensor; POC diagnostics; SARS-COV; CORONAVIRUS; BIOSENSOR; ASSAY;
D O I：
10.1002/elan.202200349
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Here, a novel biosensing platform for the detection of SARS-CoV-2 usable both at voltammetric and impedimetric mode is reported. The platform was constructed on a multi-walled carbon nanotubes (MWCNTs) screen-printed electrode (SPE) functionalized by methylene blue (MB), antibodies against SARS-CoV-2 spike protein (SP), a bioactive layer of chitosan (CS) and protein A (PrA). The voltammetric sensor showed superior performances both in phosphate buffer solution (PBS) and spiked-saliva samples, with LOD values of 5.0 +/- 0.1 and 30 +/- 2.1 ng/mL, compared to 20 +/- 1.8 and 50 +/- 2.5 ng/mL for the impedimetric sensor. Moreover, the voltammetric immunosensor was tested in real saliva, showing promising results.
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页数：10
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