Investigation on Potential Targets of Fuyuan Xingnao Decoction in the Treatment of Intracerebral Hemorrhage Based on Network Pharmacology

Fuyuan Xingnao decoction has the characteristics of multi-component, multi-pathway and multi-target. Previous studies have found that it has antioxidant, anti-inflammatory and other biological effects, and is now often used to treat neurological diseases such as intracerebral hemorrhage and cerebral infarction. But the specific key ingredients and mechanism are still unclear. Modern bioinformatics and network pharmacology methods were used to predict molecular network mechanisms between intracerebral hemorrhage and Fuyuan Xingnao decoction. Animal experiments were carried out to verify the effect of Fuyuan Xingnao decoction for the treatment of intracerebral hemorrhage, combined with behavior test and pathological detection. 68 active components in Fuyuan Xingnao decoction are involved and 471 gene targets were identified successfully. There are a total of 359 cross-targets between Fuyuan Xingnao decoction and intracerebral hemorrhage. Subsequently, the "disease-drug-active ingredient-target" network diagram was constructed using Cytoscape software and 97 core targets were screened. In addition, the enrichment of gene ontology and Kyoto Encyclopedia of Genes and Genomes suggests that phosphoinositide 3-kinase/protein kinase B and the apoptosis signaling pathway are most likely to be affected for the treatment of intracerebral hemorrhage. Finally, the use of behavioral tests and hematoxylin and eosin staining in animal experiments confirmed the role of Fuyuan Xingnao decoction in the treatment of intracerebral hemorrhage. Based on network pharmacology and experimental validation results, our in vivo study indicated that Fuyuan Xingnao decoction is effective for the treatment of intracerebral hemorrhage.