Rat Plantar Fascia Stem/Progenitor Cells Showed Lower Expression of Ligament Markers and Higher Pro-Inflammatory Cytokines after Intensive Mechanical Loading or Interleukin-1β Treatment In Vitro

被引：0

作者：

Siu, Wing Sum ^{[1
,2
]}

Ma, Hui ^{[1
,2
]}

Ko, Chun Hay ^{[1
,2
]}

Shiu, Hoi Ting ^{[1
,2
]}

Cheng, Wen ^{[1
,2
]}

Lee, Yuk Wa ^{[3
]}

Kot, Cheuk Hin ^{[3
]}

Leung, Ping Chung ^{[1
,2
]}

Lui, Pauline Po Yee ^{[3
,4
]}

机构：

[1] Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, State Key Lab Res Bioact & Clin Applicat Med Plant, Shatin, Hong Kong, Peoples R China

[3] Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Shatin, Hong Kong, Peoples R China

[4] Ctr Neuromusculoskeletal Restorat Med, Hong Kong Sci Pk, Hong Kong, Peoples R China

来源：

CELLS | 2023年 / 12卷 / 18期

关键词：

plantar fasciitis; plantar fascia stem/progenitor cells; inflammation; intensive mechanical loading; MESENCHYMAL STEM-CELLS; OSTEOGENIC DIFFERENTIATION; POTENTIAL ROLES; BONE-MARROW; TENDON; NUCLEOSTEMIN; IL-1-BETA; ETIOLOGY; IMPACT;

D O I：

10.3390/cells12182222

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The pathogenesis of plantar fasciitis is unclear, which hampers the development of an effective treatment. The altered fate of plantar fascia stem/progenitor cells (PFSCs) under overuse-induced inflammation might contribute to the pathogenesis. This study aimed to isolate rat PFSCs and compared their stem cell-related properties with bone marrow stromal cells (BMSCs). The effects of inflammation and intensive mechanical loading on PFSCs' functions were also examined. We showed that plantar fascia-derived cells (PFCs) expressed common MSC surface markers and embryonic stemness markers. They expressed lower Nanog but higher Oct4 and Sox2, proliferated faster and formed more colonies compared to BMSCs. Although PFCs showed higher chondrogenic differentiation potential, they showed low osteogenic and adipogenic differentiation potential upon induction compared to BMSCs. The expression of ligament markers was higher in PFCs than in BMSCs. The isolated PFCs were hence PFSCs. Both IL-1 beta and intensive mechanical loading suppressed the mRNA expression of ligament markers but increased the expression of inflammatory cytokines and matrix-degrading enzymes in PFSCs. In summary, rat PFSCs were successfully isolated. They had poor multi-lineage differentiation potential compared to BMSCs. Inflammation after overuse altered the fate and inflammatory status of PFSCs, which might lead to poor ligament differentiation of PFSCs and extracellular matrix degeneration. Rat PFSCs can be used as an in vitro model for studying the effects of intensive mechanical loading-induced inflammation on matrix degeneration and erroneous stem/progenitor cell differentiation in plantar fasciitis.

引用

页数：23