Continuous cultivation of the lithoautotrophic nitrate-reducing Fe(II)-oxidizing culture KS in a chemostat bioreactor

被引:0
|
作者
Bayer, Timm [1 ]
Tomaszewski, Elizabeth J. [1 ,5 ]
Bryce, Casey [2 ]
Kappler, Andreas [1 ,3 ]
Byrne, James M. [2 ,4 ]
机构
[1] Univ Tubingen, Ctr Appl Geosci, Geomicrobiol Grp, Tubingen, Germany
[2] Univ Bristol, Sch Earth Sci, Bristol, England
[3] Cluster Excellence EXC 2124 Controlling Microbes F, Tubingen, Germany
[4] Univ Bristol, Sch Earth Sci, Queens Rd, Bristol BS8 1RJ, England
[5] US Geol Survey, Boulder, CO USA
来源
ENVIRONMENTAL MICROBIOLOGY REPORTS | 2023年 / 15卷 / 04期
基金
英国科研创新办公室;
关键词
FERROUS IRON OXIDATION; FE(II) OXIDATION; MINERALIZATION PATHWAYS; CELL ENCRUSTATION; NITROUS-OXIDE; NITRIC-OXIDE; NEUTRAL PH; REDUCTION; REACTIVITY; SEDIMENTS;
D O I
10.1111/1758-2229.13149
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Laboratory-based studies on microbial Fe(II) oxidation are commonly performed for 5-10 days in small volumes with high substrate concentrations, resulting in geochemical gradients and volumetric effects caused by sampling. We used a chemostat to enable uninterrupted supply of medium and investigated autotrophic nitrate-reducing Fe(II)-oxidizing culture KS for 24 days. We analysed Fe- and N-speciation, cell-mineral associations, and the identity of minerals. Results were compared to batch systems (50 and 700 mL-static/shaken). The Fe(II) oxidation rate was highest in the chemostat with 7.57 mM Fe(II) d(-1), while the extent of oxidation was similar to the other experimental setups (average oxidation of 92% of all Fe(II)). Short-range ordered Fe(III) phases, presumably ferrihydrite, precipitated and later goethite was detected in the chemostat. The 1 mM solid phase Fe(II) remained in the chemostat, up to 15 mu M of reactive nitrite was measured, and 42% of visualized cells were partially or completely mineral-encrusted, likely caused by abiotic oxidation of Fe(II) by nitrite. Despite (partial) encrustation, cells were still viable. Our results show that even with similar oxidation rates as in batch cultures, cultivating Fe(II)-oxidizing microorganisms under continuous conditions reveals the importance of reactive nitrogen intermediates on Fe(II) oxidation, mineral formation and cell-mineral interactions.
引用
收藏
页码:324 / 334
页数:11
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