Long-term, high-resolution in vivo calcium imaging in pigeons

被引:0
|
作者
Nimpf, Simon [1 ]
Kaplan, Harris S. [2 ]
Nordmann, Gregory C. [1 ]
Cushion, Thomas [3 ]
Keays, David A. [1 ,3 ,4 ]
机构
[1] Ludwig Maximilian Univ Munich, Fac Biol, Div Neurobiol, D-82152 Planegg Martinsried, Germany
[2] Harvard Univ, Dept Mol & Cellular Biol, 16 Divin Ave, Cambridge, MA 02138 USA
[3] Univ Cambridge, Dept Physiol Dev & Neurosci, Downing St, Cambridge CB2 3EG, England
[4] Vienna Bioctr, Res Inst Mol Pathol, Campus Vienna Bioctr 1, A-1030 Vienna, Austria
来源
CELL REPORTS METHODS | 2024年 / 4卷 / 02期
基金
欧洲研究理事会;
关键词
PSYCHOPHYSICAL DETERMINATION; NUCLEUS ROTUNDUS; COLOR; ORIENTATION; DISCRIMINATION; SENSITIVITY; WAVELENGTH; SEQUENCE; UNITS; MICE;
D O I
10.1016/j.crmeth.2024.100711
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In vivo 2 -photon calcium imaging has led to fundamental advances in our understanding of sensory circuits in mammalian species. In contrast, few studies have exploited this methodology in birds, with investigators primarily relying on histological and electrophysiological techniques. Here, we report the development of in vivo 2 -photon calcium imaging in awake pigeons. We show that the genetically encoded calcium indicator GCaMP6s, delivered by the adeno-associated virus rAAV2/7, allows high -quality, stable, and long-term imaging of neuronal populations at single -cell and single -dendrite resolution in the pigeon forebrain. We demonstrate the utility of our setup by investigating the processing of colors in the visual Wulst, the avian homolog of the visual cortex. We report that neurons in the Wulst are color selective and display diverse response profiles to light of different wavelengths. This technology provides a powerful tool to decipher the operating principles that underlie sensory encoding in birds.
引用
收藏
页数:15
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