Effect of Five Different Antioxidants on the Effectiveness of Goat Semen Cryopreservation

被引:2
|
作者
Yi, Xiaohua [1 ]
Qiu, Yanbo [1 ]
Tang, Xiaoqin [1 ]
Lei, Yichen [1 ]
Pan, Yun [1 ]
Raza, Sayed Haidar Abbas [1 ,2 ,3 ]
Althobaiti, Norah A. [4 ]
Albalawi, Aishah E. [5 ]
Al Abdulmonem, Waleed [6 ]
Makhlof, Raafat T. M. [7 ]
Alsaad, Mohammad A. [8 ]
Zhang, Yu [1 ]
Sun, Xiuzhu [1 ,9 ]
机构
[1] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China
[2] South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Nation Local Joint Engn Res Ctr Machining & Safety, Guangzhou 510642, Peoples R China
[3] Shaoguan Univ, Guangdong Prov Key Lab Utilizat & Conservat Food &, Shaoguan 512005, Peoples R China
[4] Shaqra Univ, Coll Sci & Humanities, Biol Dept, Al Quwaiiyah 19257, Saudi Arabia
[5] Univ Tabuk, Fac Sci, Dept Biol, Tabuk 47913, Saudi Arabia
[6] Qassim Univ, Coll Med, Dept Pathol, POB 6655, Buraydah 51452, Saudi Arabia
[7] Umm Al Qura Univ, Fac Med, Dept Parasitol, POB 715, 21955 Mecca, Saudi Arabia
[8] Umm Al Qura Univ, Coll Med, Mecca 21955, Saudi Arabia
[9] Northwest A&F Univ, Coll Grassland Agr, Yangling, Peoples R China
关键词
Goat; Semen; Antioxidants; Cryopreservation; Oxidative damage; MITOCHONDRIA-TARGETED ANTIOXIDANT; LIPID-PEROXIDATION; OXIDATIVE STRESS; SPERM CRYOPRESERVATION; VIABILITY; PEPTIDE; LINGZHI;
D O I
10.1007/s43032-024-01452-8
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The effective combination of semen cryopreservation and artificial insemination has a positive effect on the conservation of germplasm resources, production and breeding, etc. However, during the process of semen cryopreservation, the sperm cells are very susceptible to different degrees of physical, chemical, and oxidative stress damage. Oxidative damage is the most important factor that reduces semen quality, which is affected by factors such as dilution equilibrium, change of osmotic pressure, cold shock, and enzyme action during the freezing-thawing process, which results in the aggregation of a large amount of reactive oxygen species (ROS) in sperm cells and affects the quality of semen after thawing. Therefore, the method of adding antioxidants to semen cryoprotective diluent is usually used to improve the effect of semen cryopreservation. The aim of this experiment was to investigate the effects of adding five antioxidants (GLP, Mito Q, NAC, SLS, and SDS) to semen cryoprotection diluent on the cryopreservation effect of semen from Saanen dairy goats. The optimal preservation concentrations were screened by detecting sperm viability, plasma membrane integrity, antioxidant capacity, and acrosomal enzyme activities after thawing, and the experimental results were as follows: the optimal concentrations of GLP, Mito Q, NAC, SLS, and SDS added to semen cryopreservation diluent at different concentrations were 0.8 mg/mL, 150 nmol/L, 0.6 mg/mL, 0.15 mg/ mL, 0.6 mg/mL, and 0.15 mg/mL. The optimal concentrations of the five antioxidants were added to the diluent and analyzed after 1 week of cryopreservation, and it was found that sperm viability, plasma membrane integrity, and mitochondrial activity were significantly enhanced after thawing compared with the control group (P < 0.05), and their antioxidant capacity was significantly enhanced (P < 0.05). Therefore, the addition of the above five antioxidants to goat sperm cryodilution solution had a better enhancement of sperm cryopreservation. This study provides a useful reference for exploring the improvement of goat semen cryoprotection effect.
引用
收藏
页码:1958 / 1972
页数:15
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