In Vitro Assessment of Antiviral, Antimicrobial, Antioxidant and Anticancer Activities of Ulvan Extracted from the Green Seaweed Ulva lactuca

Marine macroalgae contain a variety of bioactive substances that have many different uses. Ulvan, a sulfated hetero-polysaccharide isolated from Ulva lactuca, has been described and investigated for its antioxidant, antiviral, antibacterial, and cytotoxic action against the cell lines PC-3 and A-549. The yield of polysaccharides was 26.53 & PLUSMN; 0.32. (Algal dry weight) with 2.23% uronic acid. According to HPLC estimates, the polysaccharide isolated from Ulva lactuca contains the neutral sugars glucose, rhamnose and galactose, as well as fructose, and melibiose. The FT-IR spectrum of ulvan revealed bands at 1227.73 cm(- 1) and 1333.97 cm(- 1), respectively, indicative of sulfate esters and uronic acids. This study came to the conclusion that Ulva lactuca ulvan (ULU) contains beneficial elements that can stop the development of cancer cells. The lowest viability of PC-3 cells, which occur at the highest concentration of ULU (500 & mu;g/ml), was 17.58% with an IC50 of 237.02 & PLUSMN; 6.84 & mu;g/ml, while the lowest viability of lung carcinoma cells was 13.91% with an IC50 of 124.38 & PLUSMN; 4.78 & mu;g/ml. This indicates that the cytotoxicity against the prostate cancer cell line was lower than that against the lung carcinoma cell line. Although ULU has no activity against adenovirus, it had antiviral efficacy against HAV-10. ULU has been shown to have antibacterial efficacy against pathogenic bacteria, including Staphylococcus aureus ATCC25923 Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29,212, Pseudomonas aeruginosa ATCC 9027, Klebsiella pneumonia ATCC 13,883, Pseudomonas fluorescent ATCC13525 and Streptococcus agaloctiae ATCC13813. The polysaccharide, meanwhile, has limited antioxidant action.