Analysis of Clinical Samples of Pancreatic Cyst's Lesions with A Multi-Analyte Bioelectronic Simot Array Benchmarked Against Ultrasensitive Chemiluminescent Immunoassay

被引：4

作者：

Scandurra, Cecilia ^{[2
,3
]}

Bjorkstrom, Kim ^{[4
]}

Caputo, Mariapia ^{[5
]}

Sarcina, Lucia ^{[2
,3
]}

Genco, Enrico ^{[6
]}

Modena, Francesco ^{[7
]}

Viola, Fabrizio Antonio ^{[1
,7
]}

Brunetti, Celestino ^{[8
]}

Kovacs-Vajna, Zsolt M. ^{[9
]}

Franco, Cinzia Di ^{[10
]}

Haeberle, Lena ^{[11
,12
]}

Larizza, Piero ^{[8
]}

Mancini, Maria Teresa ^{[8
]}

Osterbacka, Ronald ^{[4
]}

Reeves, William ^{[13
]}

Scamarcio, Gaetano ^{[14
]}

Wheeler, May ^{[13
]}

Caironi, Mario ^{[7
]}

Cantatore, Eugenio ^{[6
]}

Torricelli, Fabrizio ^{[9
]}

Esposito, Irene ^{[11
,12
]}

Macchia, Eleonora ^{[4
,5
]}

Torsi, Luisa ^{[2
,3
]}

机构：

[1] Univ Cagliari, Dipartimento Ingn Elettr & Elettron, I-09123 Cagliari, Italy

[2] Univ Bari Aldo Moro, Dipartimento Chim, I-20125 Bari, Italy

[3] Univ Bari Aldo Moro, Ctr Colloid & Surface Sci, I-20125 Bari, Italy

[4] Abo Akad Univ, Fac Sci & Engn, Turku 20500, Finland

[5] Univ Bari Aldo Moro, Dipartimento Farm Sci Farmaco, I-70125 Bari, Italy

[6] Eindhoven Univ Technol, Dept Elect Engn, NL-5600 MB Eindhoven, Netherlands

[7] Ist Italiano Tecnol, Ctr Nano Sci & Technol, Via Rubattino 81, I-20134 Milan, Italy

[8] Masmec Biomed Masmec SpA Div, I-70026 Modugno, BA, Italy

[9] Univ Brescia, Dipartimento Ingn Informaz, I-25123 Brescia, Italy

[10] CNR, IFN, I-70126 Bari, Italy

[11] Heinrich Heine Univ, Inst Pathol, D-40225 Dusseldorf, Germany

[12] Univ Hosp Dusseldorf, D-40225 Dusseldorf, Germany

[13] FlexEnable Ltd, Cambridge CB4 0FX, England

[14] Univ Bari Aldo Moro, Dipartimento Interateneo Fis, I-70125 Bari, Italy

来源：

ADVANCED SCIENCE | 2024年 / 11卷 / 27期

关键词：

bioelectronic transistors; liquid biopsy; multivariate data processing; SIMOA-single-molecule-array-; SiMoT-single-molecule-with-a-large-transistor; single-molecule biosensors; SURFACE-PLASMON RESONANCE; LABEL-FREE; ASSAY; MORPHOLOGY; CRYSTAL; CANCERS; LIMITS; IUPAC;

D O I：

10.1002/advs.202308141

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Pancreatic cancer, ranking as the third factor in cancer-related deaths, necessitates enhanced diagnostic measures through early detection. In response, SiMoT-Single-molecule with a large Transistor multiplexing array, achieving a Technology Readiness Level of 5, is proposed for a timely identification of pancreatic cancer precursor cysts and is benchmarked against the commercially available chemiluminescent immunoassay SIMOA (Single molecule array) SP-X System. A cohort of 39 samples, comprising 33 cyst fluids and 6 blood plasma specimens, undergoes detailed examination with both technologies. The SiMoT array targets oncoproteins MUC1 and CD55, and oncogene KRAS, while the SIMOA SP-X planar technology exclusively focuses on MUC1 and CD55. Employing Principal Component Analysis (PCA) for multivariate data processing, the SiMoT array demonstrates effective discrimination of malignant/pre-invasive high-grade or potentially malignant low-grade pancreatic cysts from benign non-mucinous cysts. Conversely, PCA analysis applied to SIMOA assay reveals less effective differentiation ability among the three cyst classes. Notably, SiMoT unique capability of concurrently analyzing protein and genetic markers with the threshold of one single molecule in 0.1 mL positions it as a comprehensive and reliable diagnostic tool. The electronic response generated by the SiMoT array facilitates direct digital data communication, suggesting potential applications in the development of field-deployable liquid biopsy. SiMoT-Single-Molecule with Large Transistor technology simultaneously analyzes protein and genetic markers, achieving a one-molecule threshold in 0.1 mL. Benchmarking against SIMOA chemiluminescent ultrasensitive assay, SiMoT outperforms SIMOA in speed and overall performance. Moreover, SiMoT provides an electronic response, enhancing its suitability for direct digital data communication.image

引用

页数：14

共 1 条

[1] Single Molecule with a Large Transistor - SiMoT cytokine IL-6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array
Scandurra, Cecilia
Bjorkstrom, Kim
Sarcina, Lucia
Imbriano, Anna
Di Franco, Cinzia
Osterbacka, Ronald
Bolella, Paolo
Scamarcio, Gaetano
Torsi, Luisa
Macchia, Eleonora
ADVANCED MATERIALS TECHNOLOGIES, 2023, 8 (11)

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