Zinc oxide nanoparticles functionalized with curcumin supplementation during in vitro embryo culture impaired in a concentration-dependent-manner blastocyst production in cattle

被引：2

作者：

Miglio, Luisa ^{[1
]}

Novaes, Marco Aurelio Schiavo ^{[2
]}

de Lima, Laritza Ferreira ^{[1
]}

Arantes, Tatiane Moraes ^{[3
]}

Alves, Kele Amaral ^{[4
]}

Dias, Francisca Elda Ferreira ^{[5
]}

Beletti, Marcelo Emilio ^{[6
]}

de Figueiredo, Jose Ricardo ^{[1
,7
]}

机构：

[1] Univ Estadual Ceara, Fac Vet Med, Lab Manipulat Oocyte & Preantral Follicles LAMOFOP, Fortaleza, Brazil

[2] Fed Rural Univ Amazon, Paragominas, Brazil

[3] Fed Univ Jatai, Jatai, Brazil

[4] Concept Biosci Inc, Berkeley, CA USA

[5] Fed Univ Tocantins, Palmas, Brazil

[6] Univ Fed Uberlandia, Inst Biomed, Lab Reprod Biol, Uberlandia, Brazil

[7] Univ Estadual Ceara UECE, Lab Manipulacao Oocitos & Foliculos Pre Antrais LA, Programa PosGrad Ciencias Vet PPGCV, Av Prof Silas Munguba 1700, BR-60740930 Fortaleza, Brazil

来源：

REPRODUCTION IN DOMESTIC ANIMALS | 2023年 / 58卷 / 08期

关键词：

antioxidant; bovine; in vitro embryo production; DEVELOPMENTAL COMPETENCE; ZNO NANOPARTICLES; MATURATION; DELIVERY; IMPROVES; ACID;

D O I：

10.1111/rda.14416

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This work investigated the effect of zinc oxide nanoparticles functionalized with curcumin (ZnO(np) + CUR) supplementation during the in vitro embryo culture (IVC) on the bovine in vitro embryo production, and the cellular antioxidant response. The cumulus-oocyte complexes (COCs) were matured, fertilized and then the presumptive zygotes were cultured in the medium in the absence (0 & mu;M-control) or presence of different concentrations of ZnO(np) + CUR (3, 6 or 12 & mu;M). After IVC, the embryos were destined either to assay intracellular ROS levels and mitochondrial membrane potential. The results demonstrated that only the addition of 12 & mu;M ZnO(np) + CUR during IVC decreased intracellular ROS production and the rate of blastocyst production when compared to the control (p < .05). In conclusion, ZnO(np) + CUR addition during the IVC impaired in concentration-dependent-manner bovine in vitro embryo production.

引用

页码：1172 / 1175

页数：4

共 1 条

[1] Anethole supplementation during in vitro maturation increases in vitro goat embryo production in a concentration-dependent manner
Conceicao-Santos, A. L.
Ferreira, A. C. A.
Sa, N. A. R.
Palomino, G. J. Q.
Silva, A. F. B.
Oliveira, A. C.
Velarde, J. M. D. S.
Celestino, J. J. H.
Rodrigues, A. P. R.
Figueiredo, J. R.
THERIOGENOLOGY, 2024, 215 : 78 - 85

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