Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli

被引：1
|
作者：
Bruggeman, Christine W. [1 ,2 ]
Peterman, Guus J. G. [1 ,2 ]
Peterman, Erwin J. G. [1 ,2 ]
机构：
[1] Vrije Univ Amsterdam, LaserLaB, NL-1081 HV Amsterdam, Netherlands
[2] Vrije Univ Amsterdam, Dept Phys & Astron, NL-1081 HV Amsterdam, Netherlands
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 01期
基金：
欧洲研究理事会;
关键词：
Biophysics; Cell Biology; Microscopy; Model Organisms;
D O I：
10.1016/j.xpro.2023.102121
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Here, we present a protocol to use microfluidics in combination with fluorescence microscopy to expose the C. elegans tail to chemosensory stimuli. We describe steps for the preparation of microfluidic chips and sample preparation through the sedation of C. elegans. We detail flow calibration and imaging of C. elegans through fluorescence microscopy to determine their molecular and/ or cellular response to chemosensory stimuli. This protocol can also be applied to amphid neurons by inserting the worm in the chip head-first. For complete details on the use and execution of this protocol, please refer to Bruggeman et al. (2022).1
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页数：12
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