Nine years of warming and nitrogen addition in the Tibetan grassland promoted loss of soil organic carbon but did not alter the bulk change in chemical structure

Nitrogen (N) and warming effects on ecosystem carbon (C) budgets and stabilization are critical to understand as C sequestration is considered as a mechanism to offset anthropogenic CO 2 emissions, which is important for accurately predicting ecosystem C sequestration and/or potential C loss, remaining controversial though. However, the relevant information, especially for the intervention of environmental controls on grassland soil, is limited in Tibetan Plateau (TP) regions. Here we used a 9-year two-way factorial experiment involving warming with open top chambers ( + 1.80 circle C in the daytime and + 0.77 circle C in the nighttime at the soil surface) and multilevel nitrogen (N) enrichment treatments (0, 5, 10, and 15 g m - 2 yr - 1 ) in the TP to investigate the changes in soil organic carbon (SOC) pool size and chemical structure. Nine-year warming treatment significantly decreased SOC stock in the Tibetan grassland. We observed decreasing SOC concentrations which may be related to changes in the C-degrading enzymes. Surprisingly, the SOC molecular structure remained unchanged in all N-enrichment and warmed plots, suggesting that both treatments had affected all forms of SOC, from simple and complex polymeric in a similar way. Our results suggest that long-term warming stimulates soil C loss but no preference in SOC loss with different chemical structures.