Clonal dissemination of successful emerging clone mecA-MRSA t304/ST6 among humans and hedgehogs in the Helsinki metropolitan area in Finland

被引：3

作者：

Venla, Johansson ^{[1
]}

Ahmad, Al-Mustapha ^{[1
]}

Viivi, Heljanko ^{[1
]}

Laura, Lindholm ^{[2
]}

Saara, Salmenlinna ^{[2
]}

Sanna, Sainmaa ^{[3
]}

Annamari, Heikinheimo ^{[1
,4
]}

机构：

[1] Univ Helsinki, Fac Vet Med, Dept Food Hyg & Environm Hlth, Agnes Sjoberginkatu 2,POB 66, Helsinki 00790, Finland

[2] Finnish Inst Hlth & Welf, Dept Hlth Secur, Expert Microbiol Unit, Mannerheimintie 166,POB 30, Helsinki 00271, Finland

[3] Korkeasaari Zoo, Mustikkamaanpolku 12,POB 1000, Helsinki 0081, Finland

[4] Finnish Food Author, Microbiol Unit, Mustialankatu 3,POB 200, Helsinki 00790, Finland

来源：

ONE HEALTH | 2023年 / 16卷

基金：

芬兰科学院;

关键词：

Methicillin-resistant Staphylococcus aureus; Wild animals; Antimicrobial resistance; Human -wildlife interface; t304; Hedgehog; RESISTANT STAPHYLOCOCCUS-AUREUS; CASSETTE CHROMOSOME MEC; PROTEIN; PERFORMANCE; HOMOLOG; GENES;

D O I：

10.1016/j.onehlt.2023.100516

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Methicillin-resistant Staphylococcus aureus (MRSA) carrying mecC gene (mecC-MRSA) is frequently reported among European hedgehogs (Europeaus erineaus) due to co-evolutionary adaptation to dermatophyte infection in European hedgehogs. The occurrence of MRSA in European hedgehogs in Finland is unknown. Consequently, we investigated the occurrence of MRSA in wild hedgehogs from urban Helsinki metropolitan area in 2020-2021 and applied whole genome sequencing (WGS) to further characterize the studied isolates and compared them with human clinical MRSA isolates. Altogether 115 dead hedgehogs were screened for MRSA using selective cultivation methods. Presumptive MRSA isolates were tested for antimicrobial susceptibility and confirmed MRSA isolates were further characterized by spa-typing and WGS. Hedgehog derived MRSA isolates were compared with clinical human MRSA isolates using core genome multilocus sequence typing (cgMLST). In total MRSA was recovered from 11 out of 115 (10%) hedgehogs. Among these four different spa types (t304; n = 4, t8835; n = 4, t5133; n = 2 and t622; n = 1) and three different sequence types (STs) (ST6; n = 6, ST7663; n = 4 and ST2840; n = 1) were identified. From the studied MRSA isolates seven harboured the mecA gene (mecAMRSA) and four were identified as mecC-MRSA. All mecA-MRSA isolates carried immune evasion cluster genes, and one isolate was positive for Panton-Valentine leukocidin. cgMLST comparison revealed close genetic relatedness among three hedgehog and two human mecA-MRSA isolates all belonging to t304/ST6. Our results suggest a clonal dissemination of a successful MRSA clone among humans and hedgehogs. Further studies are warranted to investigate the sources and dissemination of such clone in urban environments. We observed a relatively low occurrence of mecC-MRSA in Finnish hedgehogs.

引用

页数：7

共 1 条

[1] Repeated introduction and spread of the MRSA clone t304/ST6 in northern Europe
Bartels, M. D.
Worning, P.
Andersen, L. P.
Bes, M.
Enger, H.
As, C. G.
Hansen, T. A.
Holzknecht, B. J.
Larssen, K. W.
Laurent, F.
Makitalo, B.
Pichon, B.
Svartstrom, O.
Westh, H.
[J]. CLINICAL MICROBIOLOGY AND INFECTION, 2021, 27 (02) : 284.e1 - 284.e5

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