Proliferation of intestinal crypt cells by gastrin-induced ornithine decarboxylase

AIM:to determine whether the gastrin stimulated intestinalcrypt cell(IEC-6)proliferation by induction of ornithinedecarboxylase(ODC).METHODS:IEC-6 cells were grown in DMEM containing50mL·L-1dialyzed fetal bovine serum for 24h and then weretreated with gastrin.The proliferative capability of the cellswas monitored subsequently on d 1,2,3,and 4 aftertreatment with MTT assay at aborbance 570nm.The cellularODC mRNA expression,ODC activity,and putrescinecontent were examined by RT-PCR method,radiometrictechnique and high-performance liquid chromatography(HPLC)analysis respectively after 12h of treatment.RESULTS:On d1 after exposure of IEC-6 cells topentagastrin,the proliferation increased initially and reacheda peak on d3 at 250μg·L-1concentration.Pentagastrin 500μg·L-1increased cell proliferation on day 1 and day 2,andthen decreased,Compared with control group,pentagastrin250μg·L-1increased ODC mRNA level by 1.09-fold(P<0.05),ODC activity by 1.71-fold(P<0.0]),and putrescinecontent 5.30-fold(P<0.01)respectively.Similarly,pentagastrin of 500μg·L-1also increased ODC mRNA levelby 1.16-fold(P<0.05),ODC activity 1.63-fold(P<0.05),and putrescine content 4.4t-fold(P<0.01)respectively.But there was not significant difference between them.CONCLUSION:Gastrin is an agent which promotes IEC-6 cellproliferation involved in regulating ODC activity mechanism.