Construction of human and mouse brain cDNA libraries and isolation of full-length cDNAs

被引：0
|
作者：
ZHOU Yan
机构：
来源：
Progress in Natural Science:Materials International | 2002年 / 06期
基金：
中国国家自然科学基金;
关键词：
cDNA library; brain; EST;
D O I：
暂无
中图分类号：
Q523 [脱氧核糖核酸（DNA）];
学科分类号：
071010 ; 081704 ;
摘要：
cDNA libraries from aborted human 3-month fetal brain, adult rat and mouse brain were constructed by using a XZAP express cDNA library construction kit. Low molecular weight fragments of the second strand cDNAs were removed by flowing through the Sepharose CL-4B column and the fractionated long, middle, short fragments and the combined fragments were respectively inserted into clone vectors to construct the cDNA libraries of the brain of human 3-month fetus. The 5’ ends of 1200 clones from each of human fetal brain cDNA libraries were sequenced. A total of 894 ESTs were obtained and some full-length clones were sequenced. By analyzing the sequences, 12 novel full-length cDNAs were obtained.
引用
收藏
页码：79 / 82
页数：4
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