RELATIONSHIP BETWEEN G-CSF AND HYPERLEUKOCYTOSIS INPATIENTS WITH APL AFTER TREATMENT WITH ALL-TRANSRETINOIC ACID

Objective: To detect the G-CSF effect and itsmechanism in hyperleucocytosis of patients with APLafter treatment with all-trans retinoic acid (ATRA).Methods: MTT method was used to measure theproliferation of HL-60 cells or primary APL cells.cDNA-mRNA dot blot hybridization was used to detectthe c-myc gene expression level of HL-60 cells afterATRA treatment. Cycle shift of HL-60 cells wasmeasured by flow cytometric Analysis. Results: ATRAcould inhibit obviously in vitro the proliferation of HL60 cells of primary APL cells. The G0/G, ratio of HL-60at 24 hours or 96 hours post-treatment with ATRA wasincreased, proliferation of HL-60 cells at the same timewas promoted obviously under the condition of rhGCSE C-myc gene expression was analyzed by dot blothybridization; its expression levels of 8, 24 or 96 hoursin culture with ATRA separately were higheF, low, highor lower as compared with each other. Conclusion: GCSF plays an important role in hyperleucocytosis ofpatients with APL after treatmellt with ATRA.