KAE Alleviates LPS-induced Neuroinflammation in the Striatum of Mice via Down-regulating TLR4/MyD88 Signaling Pathway

被引：1

作者：

YANG Ying-Lin ^{[1
]}

CHENG Xiao ^{[1
]}

LI Wei-Han ^{[1
]}

LIU Man ^{[1
]}

王月华 ^{[1
]}

杜冠华 ^{[1
]}

机构：

[1] Beijing Key Laboratory of Drug Target Identification and Drug Screening,Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College

来源：

神经药理学报 | 2018年 / 8卷 / 02期

关键词：

neuroinflammation; striatum; TLR4; MyD88;

D O I：

暂无

中图分类号：

R965 [实验药理学];

学科分类号：

100602 ; 100706 ;

摘要：

Objective:Kaempferol（ KAE） is a natural flavonoid with many biological activities including anti-oxidation and anti-inflammation. Nevertheless,the relevant mechanism of its antineuroinflammation remains unclear. The present study was to investigate the mechanism of KAE against LPS-induced neuroinflammation in the striatum of mice. Methods:Lipopolysaccharide（ LPS）,a lipid polysaccharide,can be used to establish animal inflammation model. Adult male BALB/c mice were treated with KAE 20 mg·kg;and 50 mg·kg;for 7 days. On the 7;day,the mice were treated with LPS 5 mg·kg;for 6 h. The levels of IL-1β,IL-6,TNF-α,MCP-1 and ICAM-1 in the striatum were determined by ELISA. The protein expression of TLR4,MyD88,HMGB1 and P2X7 in the striatum were detected by Western blotting. Results:KAE significantly decreased cytokines release including IL-1β,IL-6,TNF-α,MCP-1 and ICAM-1 in the striatum of mice induced by LPS. In addition,KAE also inhibited the inflammatory proteins expression,such as TLR4,MyD88,HMGB1 and P2X7,in the striatum of mice induced by LPS. Conclusion:KAE alleviates LPS-induced neuroinflammation in the striatum of mice via inhibiting TLR4/MyD88 signaling pathway.

引用

页码：30 / 30

页数：1