A narrative review of single-nucleotide polymorphism detection methods and their application in studies of Staphylococcus aureus

Single-nucleotide polymorphisms (SNPs) are the third generation of genetic markers, having been refined from the first generation of restriction fragment length polymorphisms and the second generation of microsatellite polymorphisms. SNPs represent a focal point of current studies ofStaphylococcus aureus. On one hand, this review aims to summarize common methodologies for detecting SNPs. These methods have typically included DNA genome sequencing methods and PCR-based detection methods. Alternative methods, such as mass spectrometry, denaturing high-performance liquid chromatography, SNaPshot, and SNP array have also been employed for SNP analysis. On the other hand, we enumerate a series of applications of SNP analysis in investigations ofStaphylococcus aureus. SNP analysis can be applied to investigate epidemiological outbreaks and transmission ofStaphylococcus aureus infections, the transmission and evolution of antimicrobial resistance genes inStaphylococcus aureus isolates, interactions ofStaphylococcus aureus with other bacteria, and the links betweenStaphylococcus aureus in humans and livestock.