Influence of Iron Supplementation on DMT1(IRE)-induced Transport of Lead by Brain Barrier Systems in vivo

被引:0
|
作者
AN Dai Zhi [1 ]
AI Jun Tao [2 ]
FANG Hong Juan [3 ]
SUN Ru Bao [1 ]
SHI Yun [1 ]
WANG Li Li [1 ]
WANG Qiang [1 ]
机构
[1] Institute of Disease Control and Prevention,Academy of Military Medical Sciences
[2] Beijing Vocational College of Agriculture
[3] Beijing Tian Tan Hospital,Capital Medical University
基金
中国国家自然科学基金;
关键词
Lead; Iron; Blood-brain barrier; Divalent metal transporter 1; MAPK pathway;
D O I
暂无
中图分类号
R114 [卫生毒理];
学科分类号
100405 ;
摘要
Objective To investigate the potential involvement of DMT1(IRE) protein in the brain vascular system in vivo during Pb exposure. Methods Three groups of male Sprague-Dawley rats were exposed to Pb in drinking water, among which two groups were concurrently administered by oral gavage once every other day as the low and high Fe treatment group, respectively, for 6 weeks. At the same time, the group only supplied with high Fe was also set as a reference. The animals were decapitated, then brain capillary-rich fraction was isolate from cerebral cortex. Western blot method was used to identify protein expression, and RT-PCR to detect the change of the m RNA. Results Pb exposure significantly increased Pb concentrations in cerebral cortex. Low Fe dose significantly reduced the cortex Pb levels, However, high Fe dose increased the cortex Pb levels. Interestingly, changes of DMT1(IRE) protein in brain capillary-rich fraction were highly related to the Pb level, but those of DMT1(IRE) m RNA were not significantly different. Moreover, the consistent changes in the levels of p-ERK1/2 or IRP1 with the changes in the levels of DMT1(IRE). Conclusion These results suggest that Pb is transported into the brain through DMT1(IRE), and the ERK MAPK pathway is involved in DMT1(IRE)-mediated transport regulation in brain vascular system in vivo.
引用
收藏
页码:651 / 659
页数:9
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