ACTIVITY STANDARD OF DONOR ENDOTHELIAL CELLS IN PENETRATING KERATOPLASTY

Penetrating keratoplasty was done in 73 patients with eyeballs enucleated from donors aged l-49 years 2-12 h.ours after death and kept in the human cord serum or in moist condition. The operation started with a grafc of diameter being 68 mm and continuous suture with 10-0 nylon thread under operating micro- scope. B.fore penetrating keratoplasty, Olympus pho- tomicroscope was used to record the number of donor en.dothelial cells but after operation contact specular photomicroscope EB-160 Model III was used Lo observe and photograph donor grafting endothelial cells for evatuating their activity density and activity ratc. After graft was taken, the remained corneal ring schcduled for observation was stained by modi- ficcl stocker’s method with trypan blue. Follow-up for 0.5-6.5 years in our 73 patients showed that using the cndothelial cell activity density as a standard for evaluating donor grafts is of clinical value in eye bank.