Microbial diversity in failed endodontic root-filled teeth

被引：0
|
作者：
ZHANG ChenHOU BenxiangZHAO Huanying and SUN Zheng School of StomatologyCapital Medical UniversityBeijing China Medical Experiment and Test CenterCapital Medical University Beijing China [100050 ,100069 ]
机构：
来源：
中华医学杂志(英文版) | 2012年 / 06期
基金：
中国国家自然科学基金;
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中图分类号：
R781.05 [治疗学];
学科分类号：
100302 ;
摘要：
Background Persistent/secondary infections of human root canals play an important role in the failure of endodontic treatment.This study used 16S rRNA sequencing to assess microbial diversity in root-filled teeth associated with failed endodontic treatment. Methods DNA was extracted from 15 teeth with persistent intraradicular infections,and the 16S rRNA of all present bacteria were amplified by PCR,followed by cloning and sequencing of the 16S rRNA amplicons.
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页码：1163 / 1168
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