Inhibitory Effect of Ferulic Acid on Oxidation of L-DOPA Catalyzed by Mushroom Tyrosinase

被引：0
|
作者：
龚盛昭 [1 ]
程江 [2 ]
杨卓如 [2 ]
机构：
[1] School of Chemical & Energy Engineering,South China University of Technology,Guangzhou 510640,China Department of Chemical Engineering,Guangdong Industry Technical College,Guangzhou 510300,China
[2] School of Chemical & Energy Engineering,South China University of Technology,Guangzhou 510640,China
来源：
Chinese Journal of Chemical Engineering | 2005年 / 06期
关键词：
tyrosinase; ferulic acid; kinetics; inhibition; L-DOPA; diphenolase;
D O I：
暂无
中图分类号：
O643.32 [催化反应];
学科分类号：
081705 ;
摘要：
The inhibitory effect of ferulic acid on the diphenolase activity of mushroom tyrosinase and the kinetic behavior were studied with L-3,4-dihydroxyphenylalanine (L-DOPA) as substrate. The inhibitor concentration leading to 50% relative activity lost (IC50) was estimated to be 0.15mmol·L-1. The inhibition mechanism obtained from Lineweaver-Burk plots shows that ferulic acid is a competitive inhibitor and the inhibition of tyrosinase by ferulic acid is a reversible reaction. The equilibrium constant for ferulic acid binding with the tyrosinase was determined to be 0 25mmol·L-1 for diphenolase.
引用
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页码：771 / 775
页数：5
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