Continuous Replication and Expression of the Hepatitis D Virus Genome in Human Hepatoma Cells

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四川大学学报(医学版) | 1993年 / 01期

关键词：

HDV; QGY; HDAg; Continuous Replication and Expression of the Hepatitis D Virus Genome in Human Hepatoma Cells;

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摘要：

With calcium phosphate method, humanhepatoma cell line of Qidong, China wascotransfected by a recombinant plasmid contain-ing full length trimer of HDV cDNA（pSVL D）and a plasmid containing neomycin-resistantgene （pSV-neo）. After selection with neomy-cin analogue G418, 2 stable G418 resistant cellclones （QGY-W911 and QGY--W913） were ob-tained. These two cell clones were detectedfor HDV cDNA by Southern blot HDV RNA by

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页码：22 / 22

页数：1