Genetic Transformation of Brassica campestris L.ssp.pekinensis via Agrobacterium-with Bt Gene

被引：0
|
作者：
Wang Feng
机构：
来源：
Agricultural Science & Technology | 2002年 / 04期
关键词：
Chinese cabbage; Bt gene; genetic transformation; cry IA(b);
D O I：
10.16175/j.cnki.1009-4229.2002.04.002
中图分类号：
S476 [生物防治];
学科分类号：
0904 ;
摘要：
Abstract: By means of Agrobacterium-mediated transformation,43 kanamycin-resistant buds ofChinese cabbage were got.PCR,PCR-Southern blot and dot blot analysis were used to identify andcharacterize the putative transgenic plants.26 plants had the predicted bands of the fragment of npt Ⅱgene.Insect bioassays of 4 transformants showed that toxic protein had been translated and thetranslation levels were different among these transformants.
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页码：10 / 14
页数：5
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