The water-soluble bicyclic 2-pyridone-based fluorescent probe for fast and selective detection of hypochlorite

As a strong oxidizer,hypochlorite (Cl O–) are widely employed as bleaching agents and disinfectants.Determination of ClO–is required to ensure bactericidal effects and avoid hazards caused by excessive residual chlorine.Herein,the derivative bicyclic 2-pyridone,namely DHIP-Py,was prepared successfully to establish a new ClO--quantitative method.The probe exhibits excellent Cl O–selectivity over other ROS and anions/cations,high sensitivity (LOD=1.32μmol/L),fast response (<5 s),and wide-p H tolerance (pH4～10).Benefit from its good water solubility,DHIP-Py is well suited for water sample analysis and has been successfully applied to detect Cl O–in real-world food and environmental samples,including tap water,bottled water and river water.The detection results were essentially identical to that of obtained from traditional DPD method.Moreover,visual detection of Cl O–via filter paper-based solid sensor and imaging of ClO-in Escherichia coli were also achieved by DHIP-Py.These satisfactory results demonstrate that this bicyclic 2-pyridone-based hypochlorite probe is a promising free chlorine chemosensor with great potential for analytical applications.