Expression, Characterization and Antimicrobial Ability of a Variant T4 Lysozyme in Pichia pastoris

被引：0
|
作者：
Ning SUN [1 ]
Sanfeng CHEN [2 ]
Xiangming XIE [3 ]
Yueju WANG [4 ]
Gangqiang LI [1 ]
Nan WANG [1 ]
Dehu LIU [1 ]
机构：
[1] Biotechnology Research Institute, Chinese Academy of Agricultural Sciences
[2] Key Laboratory for Agrobiotechnology, China Agricultural University
[3] Department of Microbiology, College of Biological Sciences and Biotechnology, Beijing Forestry University
来源：
Agricultural Science & Technology | 2014年 / 15卷 / 03期
关键词：
Antibacterial activity; Expression; Pichia pastoris; T4; lysozyme; Variant;
D O I：
10.16175/j.cnki.1009-4229.2014.03.001
中图分类号：
Q936 [微生物生物化学];
学科分类号：
071010 ;
摘要：
T4 lysozyme was engineered with disulfide bonds and expressed in Pichia pastoris. The secreted proteins were purified and made into powder by lyophilization. Recombinant protein purity was more than 70% measured by HPLC. The lytic activity of variant T4-lysozyme was measured by the lysis of the cell wall of Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, Ralstonia solanacearum comb.nov, Clavibacter michiganensis subsp. michiganensis, X. campestris pv. malvacearum, Fusarium oxysporium sp. vasinfectum, Verticillium dahliae kleb. Inhibition zone assay showed that variant T4 lysozyme significantly inhibited X. o. oryzicola and X. c. malvacearum. The antifungal activities of this protein against F. o. vasinfectum and V. d. kleb were also analyzed.
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收藏
页码：321 / 325
页数：5
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