Inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma cells in vitro

AIM:To study the inhibitory effects of antisense RNA ofHAb18G/CD147 on invasion of hepatocellular carcinoma(HCC)cells in vitro.METHODS:Antisense RNA of HAb18G/CD147 vector PCI-asHAb18G was constructed by reversely inserting HAb18G/CD147 cDNA to eukaryotic expression vector PCI-neo.TheHCC cell line HHCC was transfected by PCI-asHAb18G viacation liposome.Expression of HAb18G/CD147 of transfectedcells selected by G418(geneticin)was observed by immuno-histochemical SP staining and FACS(fluorescence activatedcell sorting).Gelatin zymography was used to determinethe effect of PCI-asHAb18G on reducing secretions of MMP-2 and MMP-9 of the transfected cells.Boyden chamber wasemployed to test the invasion of HCC cells in vitro.RESULTS:The construction of antisense RNA vector PCI-asHAb18G was verified correct by partial nucleotidesequencing and restricted endonuclease digestion.Theexpression of HAb18G/CD147 in transfected HHCC wasinhibited by PCI-asHAb18G.Secretions of MMP-2 and MMP-9 of transfected HHCC were reduced and the invasion oftransfected HHCC was inhibited compared to HHCC,respectively.CONCLUSION:Invasion of HCC cells can be inhibited byantisense RNA of HAb18G/CD147.HAb18G/CD147 may beused as a potential target of drugs for anti-invasion andmetastasis of HCC.