MULTIPLE MUTATIONS ON DOUBLE-STRANDED DNA

被引：0

作者：

朱榴琴

申同健

机构：

[1] Academia Sinica

[2] Beijing 100080

[3] PRC

[4] Institute of Biophysics

来源：

Science Bulletin | 1990年 / 20期

关键词：

double-stranded DNA; multiple mutation; oligonucleotide primer;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The site-specific mutagenesis of the gene has become an important technique in gene modification and protein engineering. Among all methods, the primer extension one using single-stranded DNA (such as the infective form of the M13 phage ) as template and the gapped stranded one are commonly used. But some genes, especially those large

引用

页码：1729 / 1733

页数：5

共 50 条

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