Five new genera are reported, namely Rathalos Lin & Li, gen. nov., of Anyphaenidae Simon, 1892, with Anyphaena xiushanensis Song & Zhu, 1991(♂♀, China:Hubei, Sichuan) as the type species, Funny Lin & Li, gen. nov., of Dictynidae O. PickardCambridge, 1871, with Funny valentine Lin & Li, sp. nov.(♂♀, China: Sichuan) as the type species, Pararana Lin & Li, gen. nov., of Leptonetidae Simon, 1890, with Pararana gaofani Lin & Li, sp. nov.(♂, China: Jiangsu) as the type species, and Burmalema Zhao & Li, gen. nov.and Milema Zhao & Li, gen. nov. of Telemidae Fage, 1913, with Burmalema shan Zhao & Li,sp. nov.(♂♀, Myanmar) and Milema nuichua Zhao & Li, sp. nov.(♂♀, Vietnam) as the type species. Another twenty-two new species are reported in the current paper, including Araneidae Clerck, 1757: Ordgarius bo Lin & Li, sp. nov.(♀, China: Yunnan), O. liyuanba Lin & Li, sp.nov.(♀, China: Hainan) and Paraplectana magnaculata Lin & Li, sp. nov.(♀, China: Hunan);Ctenidae Keyserling, 1877: Amauropelma gui Lin & Li, sp. nov.(♂, China: Guangxi);Filistatidae Simon, 1864: Pholcoides erebus Lin & Li, sp. nov.(♂, China: Tibet); Gnaphosidae Banks, 1892: Cryptodrassus beijing Lin & Li, sp. nov.(♂♀, China: Beijing); Halonoproctidae Pocock, 1901: Latouchia yuanjingae Lin & Li, sp. nov.(♂♀, China: Hainan); Liocranidae Simon, 1897: Paratus meiyingae Lin & Li, sp. nov.(♂, China: Yunnan) and P. nanling Lin &Li, sp. nov.(♂♀, China: Guangdong); Ochyroceratidae Fage, 1912: Speocera tongyaoi Lin &Li, sp. nov.(♂♀, China: Guangdong) and S. wangi Lin & Li, sp. nov.(♂, China: Hainan);Oxyopidae Thorell, 1869: Hamataliwa wangi Lin & Li, sp. nov.(♂♀, China: Fujian); Salticidae Blackwall, 1841: Hyllus shanhonghani Lin & Li, sp. nov.(♂♀, China: Jiangxi); Selenopidae Simon, 1897: Siamspinops banna Lin & Li, sp. nov.(♂♀, China: Yunnan); Telemidae Fage,1913: Mekonglema chiangmai Zhao & Li, sp. nov.(♂♀, Thailand), Milema lorkor Zhao & Li,sp. nov.(♂♀, Thailand) and M. sai Zhao & Li, sp. nov.(♂♀, Thailand); Tetrablemmidae O.Pickard-Cambridge, 1873: Singaporemma shenzhen Lin & Li, sp. nov.(♂♀, China:Guangdong); Tetragnathidae Menge, 1866: Dolichognatha yue Lin & Li, sp. nov.(♂♀, China:Guangdong); Theridiidae Sundevall, 1833: Spheropistha pingshan Lin & Li, sp. nov.(♂♀,China: Guangdong); Theridiosomatidae Simon, 1881: Wendilgarda housaiyuae Lin & Li, sp.nov.(♂♀, China: Yunnan) and Titanoecidae Lehtinen, 1967: Pandava banna Lin & Li, sp. nov.(♂♀, China: Yunnan). Two new combinations in the family Anyphaenidae are proposed:Rathalos treecko(Lin & Li, 2021), comb. nov., R. xiushanensis(Song & Zhu, 1991), comb.nov., both transferred from Anyphaena Sundevall, 1833.