Prognostic significance of epidermal growth factor-like domain 7 in pancreatic cancer

BACKGROUND: Recent studies have shown the clinical significance of epidermal growth factor-like domain 7(EGFL7)in a variety of cancers. However, the relationship between EGFL7 and the prognosis of pancreatic cancer(PC) remains unclear. The present study was undertaken to investigate the role of EGFL7 in the prognosis of PC.METHODS: The expression of EGFL7 in nine PC cell lines was first determined by Western blotting analysis. Tissue microarray-based immunohistochemical staining was performed in paired formalin-fixed paraffin-embedded tumor and non-tumor samples from 83 patients with PC. Finally,correlations between EGFL7 expression and clinicopathological variables as well as overall survival were evaluated.RESULTS: EGFL7 was widely expressed in all PC cell lines tested.EGFL7 expression in tumor tissues was significantly higher than that in non-tumor tissues(P0.040). In addition, univariate analysis revealed that high EGFL7 expression in tumor tissues was significantly associated with poor overall survival,accompanied by several conventional clinicopathological variables, such as gender, histological grade and lymph node metastasis. In a multivariate Cox regression test, EGFL7 expression was identified as an independent marker for longterm outcome of PC.CONCLUSION: Our data showed that EGFL7 is extensively expressed in PC and that EGFL7 is associated with poor prognosis.