Bioaugmentation of DDT-contaminated soil by dissemination of the catabolic plasmid pDOD

被引:8
|
作者
Chunming Gao [1 ]
Xiangxiang Jin [1 ]
Jingbei Ren [1 ]
Hua Fang [1 ]
Yunlong Yu [1 ]
机构
[1] Institute of Pesticide and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University
关键词
Dichlorodiphenyltrichloroethane; Bioaugmentation; Plasmid transfer; Soil; Green fluorescent protein;
D O I
暂无
中图分类号
X53 [土壤污染及其防治];
学科分类号
082803 ; 120405 ;
摘要
A plasmid transfer-mediated bioaugmentation method for the enhancement of dichlorodiphenyltrichloroethane(DDT) degradation in soil was developed using the catabolic plasmid p DOD from Sphingobacterium sp. D-6. The p DOD plasmid could be transferred to soil bacteria, such as members of Cellulomonas, to form DDT degraders and thus accelerate DDT degradation. The transfer efficiency of p DOD was affected by the donor, temperature,moisture, and soil type. Approximately 50.7% of the DDT in the contaminated field was removed 210 days after the application of Escherichia coli TG I(p DOD-gfp). The results suggested that seeding p DOD into soil is an effective bioaugmentation method for enhancing the degradation of DDT.
引用
收藏
页码:42 / 50
页数:9
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