Zinc binds to and directly inhibits protein phosphatase 2A in vitro

被引：0

作者：

Yan Xiong ^{[1
,2
,3
,4
]}

Dan-Ju Luo ^{[4
]}

Xiu-Lian Wang ^{[4
]}

Mei Qiu ^{[4
]}

Yang Yang ^{[4
]}

Xiong Yan ^{[4
]}

Jian-Zhi Wang ^{[4
]}

Qi-Fa Ye ^{[1
,2
,3
,5
]}

Rong Liu ^{[4
,6
]}

机构：

[1] Wuhan University

[2] Zhongnan Hospital of Wuhan University

[3] Institute of Hepatobiliary Diseases of Wuhan University,Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation

[4] Key Laboratory of Neurological Disease, Ministry of Education, and Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology

[5] The Third Xiangya Hospital of Central South University, Research Center of National Health Ministry on Transplantation Medicine Engineering and Technology

[6] The Institute of Brain Research, Huazhong University of Science and Technology

来源：

Neuroscience Bulletin | 2015年 / 31卷 / 03期

基金：

中国国家自然科学基金;

关键词：

zinc; protein phosphatase 2A; direct inhibition;

D O I：

暂无

中图分类号：

R346 [];

学科分类号：

摘要：

Zinc induces protein phosphatase 2A(PP2A) inactivation and tau hyperphosphorylation through PP2A(tyrosine 307) phosphorylation in cells and the brain, but whether Zn2+ has a direct inhibitory effect on PP2 A is not clear. Here we explored the effect of Zn2+ on PP2 A and their direct interaction in vitro. The results showed that Zn2+ mimicked the inhibitory effect of okadaic acid on protein phosphatase and prevented tau dephosphorylation in N2 a cell lysates. PP2 A activity assays indicated that a low concentration(10 μmol/L) of Zn2+ inhibited PP2 A directly. Further Zn2+-IDA-agarose affinity binding assays showed that Zn2+ bound to and inhibited PP2Ac(51-270) but not PP2Ac(1-50) or PP2Ac(271-309). Taken together, Zn2+ inhibits PP2 A directly through binding to PP2Ac(51-270) in vitro.

引用

页码：331 / 337

页数：7

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