Associations between polymorphisms of endothelial nitric oxide synthase(eNOS) gene G298A, matrix metalloproteinase-3(MMP-3) gene 5A/6A, angiotensinogen(AGT) gene M235T,angiotensin Ⅱ type 1 receptor(AT1R) gene A1166C and risk of restenosis after percutaneo

OBJECTIVE Previous studies had provided controversial results regarding the restenosis risk for eNOS, MMP-3, AGT and AT1 R polymorphisms. The aim of our study was to summarize the relationship between those polymorphisms and risk of restenosis after percutaneous coronary intervention. METHODS Al studies published on the association of e NOS G298 A, MMP-3 5 A/6 A, AGT M235 T and AT1 R A1166 C polymorphism with restenosis were identified by searching the Pub Med, Embase,Cochrane’s Library and clinicaltrials.gov. Two authors independently selected studies, assessed the risk of bias（Newcastle-Ottawa Scale） and extracted data. The metaanalysis was performed in Stata 12.0. PROSPERO registration number: CRD 42019135173. RESULTS A total of 18 researches were analyzed in the meta-analysis, including 5 on G298 A, 4 on 5 A/6 A, 6 on M235 T and 7 on A1166 C.Overall, under several models, there were significant associations of elevated restenosis risk for e NOS gene G298 A, MMP3 gene 5 A/5 A, AGT gene M235 T and A1 TR gene A1166 c polymorphism. For G298 A variant eNOS gene, OR;=1.685（1.269, 2.338）, OR;=2.144（1.490, 3.085）, OR;=2.078（1.462, 2.954） and OR;=0.496（0.348, 0.706）. For 5 A/6 A variant MMP3 gene, OR;=0.839（0.722, 0.975）, OR;=0.846（0.733, 0.976） and OR;=1.131（1.001, 1.301）. For M235 T variant of AGT gene with OR;=1.594（1.179, 2.155）, OR;=1.437（1.077, 1.918） and OR;=0.694（0.555, 0.869）. Moreover, positive results were observed in AT1 R gene A1166 C polymorphism under three models with OR;=2.009（1.433, 2.816）,OR;=1.874（1.353, 2.595） and ORdominant=1.350（1.105, 1.649）. There were heterogeneity ranging from low（0.0%） to high（66.2%） levels found during the analysis process, in which ethnicity and intervention type might play a role. CONCLUSION The pooled OR of the present meta-analysis suggested evidence that there was an increase in the risk of restenosis conferred by the G298 A variant of e NOS gene in Caucasian, the 5 A/6 A variant of MMP-3 gene in Asian, the M235 T variant of the AGT gene in Caucasian and the A1166 C variant of A1 TR gene in Asian.