Interaction between poly(amidoamine) dendrimers and DNA studied by spectroscopic methods

被引：0
|
作者：
WANG Yanming
机构：
来源：
Science Bulletin | 2005年 / 19期
关键词：
poly(amidoamine) dendrimers; DNA; interaction; spec-troscopic methods;
D O I：
暂无
中图分类号：
Q523 [脱氧核糖核酸（DNA）];
学科分类号：
071010 ; 081704 ;
摘要：
The interaction between amino-terminated, and ethylenediamine core poly(amidoamine) (PAMAM) den-drimers and herring sperm DNA was investigated by various spectroscopic methods including UV spectroscopy, fluores-cence spectroscopy, microscopic FTIR- and circular dichro-ism (CD-) spectroscopy. Ethidium bromide (EB) is used as a nucleic acid probe for this study. Experimental results show that PAMAM dendrimers can form stable complexes with DNA and the dendrimers bind to DNA sufficiently strong which cannot be displaced by EB, and we also found that the formation of the complexes can cause the conformation change of the DNA secondary structure. According to the Scatchard analysis, the association constant of PAMAM to DNA is calculated to be 2.53 × 104 mol/L?1.
引用
收藏
页码：43 / 47
页数：5
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