Expresion Preference of the S-adenosylmethionine Synthetase(Sam-S) Gene in Drosophila melanogaster

被引：0

作者：

张靖溥 ^{[1
]}

机构：

[1] 中国科学院发育生物学研究所

来源：

Developmental and Reproductive Biology | 1997年 / 01期

关键词：

S adenosylmethionine synthetase; Drosophila melanogaster; gene expression pattern; ovary; Sam S gene;

D O I：

暂无

中图分类号：

Q952.6 [动物细胞生物化学];

学科分类号：

071010 ;

摘要：

Ｓ－腺苷甲硫氨酸合成酶（ＳａｍＳ）是目前已知的唯一在生物体内催化腺苷甲硫氨酸合成的酶。它是除自身以外、所有甲基化反应的甲基供体，并且参与多胺的生物合成。多胺对于稳定ＤＮＡ、ＲＮＡ和蛋白质大分子的双螺旋结构具有重要作用，和ＤＮＡ的甲基化一起参与了基因组的印迹（ｉｍｐｒｉｔｉｎｇ）过程。在分离到ＳａｍＳ基因的基础上，本文通过Ｎｏｒｔｈｅｒｎｂｌｏｔ和酶活两种方法，对该基因在野生型果蝇和四个等位突变体发育过程中主要阶段的转录和转译水平进行了测定。野生型果蝇由瑞典Ｕｍｅａ大学果蝇中心提供。由于该基因的突变是阴性致死突变，研究中采用了杂合子突变体：Ｓｕ（ｚ）５，Ｌ（２）Ｍ６，Ｌ（２）Ｒ２３和Ｄｆ（２Ｌ）ＰＭ４４，均由所在实验室诱变获得。Ｎｏｒｔｈｅｒｎ分析时，以ｃＤＮＡ＃１０和ａ微管蛋白基因为探针，分析果蝇卵巢、幼虫、蛹、胚胎、雄性和雌性成蝇中该基因Ｐｏｌｙ（Ａ）ＲＮＡ的转录水平。通过测定蛋白粗提物中的酶活，分析果蝇卵巢、幼虫、蛹、以及雄性成蝇腹部组织中该基因的翻译水平。Ｆｉｇ．１，２＆３表明：ＳａｍＳ基因主要在成熟雌蝇的卵巢中高表达，在雄性成蝇中该基因的表达水平明显低于雌性。在其它发育阶段及组织部位中仅维持

引用

页码：7 / 16

页数：10

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