Pandanus amaryllifoliusleaf extract improves insulin sensitivity in high-fat diet-induced obese mice

OBJECTIVE To investigate the effect of Pandanus amaryllifolius leaf in high-fat diet-induced insulin resistance in mice model.METHODS To induce obesity,male ICR mice were fed with a high-fat diet(45%fat)for six weeks.The mice were divided into four groups(n=8):non-obese control mice were treated with 5% gum arabic and obese mice were treated with Pandanus amaryllifolius(125and 250mg·kg-1·d-1),or 5% gum arabic.After six weeks of treatments,the fasting blood glucose,serum insulin,OGTT and fat cell protein expression of glucose transporter 4(GLUT4)were determined.RESULTS Administration of Pandanus amaryllifolius showed significantly(P<0.05)reduced the high blood glucose,inhibited the abnormal increase in blood glucose level during OGTT,and decreased the high level of serum insulin.Moreover,it is interesting that the protein expression of GLUT4 was effectively increased by Pandanus amaryllifolius.CONCLUSION These findings demonstrate that the extract from Pandanus amaryllifolius leaf possesses antihyperglycemic action in obese mice by improving insulin sensitivity and stimulating GLUT4 expression in adipose tissue.