1,25（OH）2D3对HL-60细胞的抑制增殖和诱导分化作用

被引：2

作者：

严舫

程涛

机构：

[1] 长海医院血液科

来源：

第二军医大学学报 | 1990年 / 03期

基金：

上海市自然科学基金;

关键词：

1,25-二羟维生素D3[1,25(OH)2D3]; HL-60细胞; 抑制增殖; 诱导分化;

D O I：

10.16781/j.0258-879x.1990.03.007

中图分类号：

学科分类号：

摘要：

本文报道1,25（OH）2D3对人早幼粒细胞白血病细胞系HL-60的抑制增殖和诱导分化作用。在10-9～10-6mol/L浓度范围内,活细胞计数及克隆形成率显示:1,25（OH）2D3对HL-60细胞的抑制增殖作用具有时间和剂量依赖性,10-7mol/L 1,25（OH）2D3的克隆形成抑制率为74.82±11.1％。从细胞形态、细胞化学、细胞表面抗原表达以及NBT还原试验等几方面,表明1,25（OH）2D3可诱导HL-60细胞向单核细胞部分分化。结合流式细胞分析结果,讨论了细胞增殖与分化的关系。

引用

页码：213 / 216+293 +293-294

页数：6

共 7 条

[1] 1,25-Dihydroxyvitamin D3-induced differentiation in a human promyelocytic leukemia cell line （HL60） : receptor-mediated maturation to macrophage-like cells. Mangelsdorf DJ,et al. The Journal of Cell Biology . 1984
[2] 1,25-Dihydroxyvitamin D3: in vivo and in vitro effects on hurnan preleukemic and leukemic cells. Koonler HP. Cancer Treatment Reports . 1985
[3] Reversibility of vitamin D-induced human leukemia cell line maturation. Bar Shavit Z,et al. The Journal of Endocrinology . 1986
[4] The effect of 1,25-dihydroxyvitamin D3 on the relationship between growth and differentiation in HL60 cells. Daniel CP,et al. Leukemia Research . 1987
[5] 1,25-Dihydroxyvitamin D, induces differentiation of human myeloid leukemia cells. Miyaura C,et al. Biochemical and Biophysical Research Communications . 1981
[6] Cell kinetics of the K-562 cells line in microculture. Jinghua Wan,Morley AA. International Journal of Cell Cloning . 1985
[7] Leukemic cell maturation: phenotypic variability and oncogene expression in HL60 cells: A review. Leglise MC,et al. Blood Cells . 1988

← 1 →